FastQCFastQC Report
Mon 6 Jun 2016
ERR1379093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873947
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCC32510.3719905211643269Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCCGTC31870.3646674226240264Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2900.041.1161943
ATGCCGT3500.034.71048744
TGCCGTC3700.032.83424845
TCTCGTA6850.023.97548141
CTCGTAT6900.023.80174442
GTATGCC7150.023.59881845
TCGTATG7100.023.13127343
AGACCGT7100.023.12994828
CCGTCAT7200.022.81261631
CGTATGC7200.022.81000544
ACCGTCA7400.022.4975430
GACCGTC7600.021.90549929
CGGAAGA7800.021.6595151
AATCTCG7750.021.4815239
GAGACCG7750.021.47906327
CGTCATC7650.021.47069732
ATCTCGT8050.020.40149540
ATCAATC8350.019.9379436
CCGAGAC8600.019.35613325
TCAATCT9200.018.82946437
Produced by FastQC (version 0.11.5)