FastQCFastQC Report
Mon 6 Jun 2016
ERR1379092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389805
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACTACTATCTCGTATGCCGTC36790.2647133950446286Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTACTATCTCGTATGCC35470.2552156597508284Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4350.034.13102743
ATGCCGT5150.028.82911944
TGCCGTC5200.028.55191445
CGGAAGA7700.021.663431
TCTCGTA7500.020.99575241
CCGACTA7700.020.74628831
CGACTAC7800.020.4803132
TATCTCG7700.020.45040939
ACCGACT7850.020.34693330
CGTATGC8000.020.24590344
CTCGTAT7800.020.18822542
AGACCGA8300.019.51342228
TCGTATG8250.019.35971843
GACCGAC8350.019.12855529
GAGACCG9000.018.9947927
GACTACT8850.018.55890733
GTATGCC8650.018.46447245
ATCTCGT8700.018.35835540
GAGCGGT11350.017.6650243
TACTATC9300.017.4177136
Produced by FastQC (version 0.11.5)