FastQCFastQC Report
Mon 6 Jun 2016
ERR1379091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936605
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATACTATCTCGTATGCCGTC25140.26841624804480013Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATACTATCTCGTATGCC20160.2152454876922502Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3150.035.71017543
ATGCCGT3400.033.74611744
TGCCGTC3800.030.19389345
CTCGTAT5250.021.85462839
TCTCGTA5250.021.85462838
CGTATGC5200.021.63212441
CCGCATA5250.021.42610528
CGCATAC5350.021.0267429
TCGTATG5400.020.83093640
TATCTCG5700.020.13033736
CGGAAGA5550.019.481141
ATCTCGT5950.019.28452537
TGAACCG706.9900684E-419.28246710
GCATACT5900.019.0666230
GAGACCG6300.018.92639424
AGACCGC5950.018.90538825
GACCGCA5950.018.90437926
AATGCCG7500.018.89681618
GTATGCC6050.018.59290142
ACCGCAT6050.018.59190827
Produced by FastQC (version 0.11.5)