FastQCFastQC Report
Mon 6 Jun 2016
ERR1379090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977317
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACACTATCTCGTATGCCGTC48830.4996331794085236Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACACTATCTCGTATGCC36140.36978789891099817Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13930.14253307780382415Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6250.037.7954543
ATGCCGT6600.036.13200844
TGCCGTC6850.035.1417545
TCGTATG9350.025.50494840
CGTACAC9600.025.3094529
CGTATGC9350.025.26433641
CTCGTAT9350.025.26433639
TATCTCG9600.025.07510236
CCGTACA9750.024.92007328
TCTCGTA9700.024.81659738
GACCGTA9800.024.7916626
ACCGTAC9950.024.4179227
AGACCGT10050.024.17619125
ATCTCGT10100.023.83376137
GTATGCC10000.023.62215242
GTACACT10400.023.36256830
TACACTA10400.023.36256831
ACTATCT10850.022.39361434
GAGACCG11050.022.19189524
AATGCCG14150.022.09867918
Produced by FastQC (version 0.11.5)