FastQCFastQC Report
Mon 6 Jun 2016
ERR1379089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785546
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCC43540.5542641678526783Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGTATATCTCGTATGCCGTC35040.44605917412856794Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4700.035.89734643
ATGCCGT5200.032.87829244
TGCCGTC5500.030.67591545
CGGAAGA7050.025.2497061
TCTCGTA8000.020.80849538
TATCTCG8200.020.57661836
CTCGTAT8100.020.55160139
GAAGAGC8800.020.4766793
CGTATGC8150.020.42551841
TCGTATG8150.020.42551840
CGCTGTA8400.020.35322629
ATCTCGT8450.019.96784237
ACCGCTG8600.019.87989627
CCGCTGT8500.019.84912328
AGACCGC8900.019.46254725
GTATGCC8600.019.3567442
GACCGCT8850.019.31831726
GAGACCG9050.018.89139224
GTATATC9150.018.44024833
CTGTATA9400.018.18914834
Produced by FastQC (version 0.11.5)