FastQCFastQC Report
Mon 6 Jun 2016
ERR1379088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893768
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCC40830.4568299603476518Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCCGTC33460.37437008261651794Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4500.038.99500344
TATGCCG4600.038.63635643
TGCCGTC5100.035.28959745
CGGAAGA8800.027.9047811
GACCGTA9850.024.43986329
CCGTACT9800.024.3363431
CGTACTA9800.024.3363432
ACCGTAC9800.024.3349830
TTCCGAT651.5254309E-524.22630920
GAAGAGC10500.023.809313
CTCGTAT9800.023.6449342
TCTCGTA9850.023.52490641
CGTATGC10050.023.50445244
TCGTATG10000.023.39700543
GTACTAC10200.023.38197533
GTATGCC10300.022.71553845
AGACCGT10650.022.60400428
ATCTCGT10500.022.06860440
CATCTCG10500.022.06860439
GAGACCG11100.021.88663927
Produced by FastQC (version 0.11.5)