FastQCFastQC Report
Mon 6 Jun 2016
ERR1379087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473060
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCATATCTCGTATGCC57791.221620936033484Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCATATCTCGTATGCCGTC48351.0220690821460279Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT7450.15748530841753688Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7020.14839555236122268Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT6350.037.2017544
TGCCGTC6350.037.2017545
TATGCCG6450.036.6249843
TGAACCG300.005148901629.99443410
TAGCTCG300.005148901629.99443421
CGGAAGA10250.028.5765971
GAAGAGC11000.026.6169193
TTCCGAT450.001226814224.99536120
GGAAGAG12200.024.0014042
TCTCGTA12150.022.9611341
TCGTATG12150.022.9611343
CTCGTAT12300.022.68111442
CGTATGC12350.022.58928944
GTATGCC12500.022.31821845
GAGACCG12900.021.97266627
AGACCGC12900.021.97266628
TATCTCG12700.021.9667539
GACCGCA12700.021.96442829
ATCTCGT12750.021.88060440
ACCGCAT12800.021.7928330
Produced by FastQC (version 0.11.5)