FastQCFastQC Report
Mon 6 Jun 2016
ERR1379085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997596
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGATAATCTCGTATGCC41570.41670175100942664Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGATAATCTCGTATGCCGTC38310.3840231917529741Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.037.6807443
ATGCCGT4650.032.4135444
TGCCGTC5050.029.8461345
CTCGATT406.135937E-428.1481763
CGGAAGA8300.026.8702471
TCGTATG8950.024.38110243
CGTAGAT9150.024.09766432
GAAGAGC9450.024.0674363
CTCGTAT9100.023.97921642
GACCGTA9200.023.96669829
CGTATGC9150.023.8481844
GTATGCC9350.023.81925645
ACCGTAG9300.023.46706430
TCTCGTA9300.023.46353341
CCGTAGA9350.023.3415731
AGACCGT9650.022.61592328
AATCTCG9950.021.93073839
CCGAGAC10150.021.7224225
GATAATC10200.021.61593836
ATCTCGT10200.021.61377140
Produced by FastQC (version 0.11.5)