FastQCFastQC Report
Mon 6 Jun 2016
ERR1379084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757607
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATATGAATCTCGTATGCCGTC37830.4993354074077985Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATGAATCTCGTATGCC33610.4436337045460245Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8700.11483526419370466Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5300.040.32510443
ATGCCGT5900.036.22424344
TGCCGTC6550.033.31640645
TCGTATG9050.023.86439340
CGTATGC9000.023.74700241
CGATATG9150.023.6035829
ACCGATA9150.023.60202227
CTCGTAT9250.023.5916239
CCGATAT9200.023.47375128
TCTCGTA9200.023.47375138
GACCGAT9550.023.08456826
GAGACCG9400.022.97430824
AGACCGA9450.022.8527525
GTATGCC9500.022.73397442
AATCTCG9650.022.6137336
CGGAAGA7400.022.5283871
CTTCGTA702.7026763E-522.4986487
ATCTCGT9650.022.38059837
AATGCCG12850.021.88295418
AGCGGTT13300.021.5049634
Produced by FastQC (version 0.11.5)