FastQCFastQC Report
Mon 6 Jun 2016
ERR1379083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924666
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTATAGAATCTCGTATGCCGTC33530.3626174207767994Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATAGAATCTCGTATGCC28410.30724607588037195Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4650.035.80085843
ATGCCGT4650.035.31706244
TGCCGTC5100.033.0830745
GACCGTA6600.024.54029526
CTCGTAT6750.024.32953339
CGTATAG6800.024.15063929
TCGTATG6800.024.15063940
CCGTATA6850.023.97435628
ACCGTAT6850.023.9730627
CGTATGC6900.023.80062941
TCTCGTA7000.023.4593538
AGACCGT7050.022.97513425
AATCTCG7300.022.80712136
ATCTCGT7400.022.1948837
GAGACCG7550.021.45360224
GTATGCC7950.020.94012542
GTATAGA8000.020.80924830
AGCGGTT9750.020.5552624
CGGAAGA5950.020.0714471
AAGAGCG10150.019.9800381
Produced by FastQC (version 0.11.5)