FastQCFastQC Report
Mon 6 Jun 2016
ERR1379082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586698
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA300.005146228729.99880830
CTCGTAT453.488909E-529.99880642
CGTCTAC507.154464E-526.99892632
CCGTCTA507.154464E-526.99892631
CCGAGAC507.154464E-526.99892625
AGAGCGG551.3650849E-424.5507555
GACCGTC551.3674567E-424.5444829
GAGCGGT702.6955786E-522.5048586
CGGAAGC500.002263821722.49910525
ATCTCGT602.4661754E-422.49910540
CGTATGC602.4661754E-422.49910544
ACCGTCT602.4661754E-422.49910530
CATCTCG754.599111E-520.99916539
AGCGGTT754.599111E-520.9991657
AGACCGT706.9797615E-419.28494628
GAGACCG600.006508278718.74925427
TCTCGTA750.00110963917.99928341
GCGGTTC800.001709952316.8743298
CGGTTCA952.8172586E-416.5782899
CGGAAGA1056.016844E-415.00451851
Produced by FastQC (version 0.11.5)