FastQCFastQC Report
Mon 6 Jun 2016
ERR1379080.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379080.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723191
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTT801.0004442E-1030.9355057
ACCGGAT751.7025741E-929.99806230
CGGATAT751.7025741E-929.99806232
CCGGATA852.0372681E-1029.11576831
GACCGGA803.3924152E-928.12318629
CGTATGC856.4774213E-926.4688844
GCGGTTC957.421477E-1026.0509518
ATCTCGT901.1903467E-824.99838640
AGACGCC450.001226575924.99838616
TCGTATG901.1903467E-824.99838643
CGGTTCA1001.3442332E-924.7484029
AGATCGC551.3629874E-424.5574553
CTCGTAT952.1142114E-823.68268242
GAGCGGT1152.6375346E-1023.486496
CGGAAGA1204.474714E-1022.5111
AGACCGG1003.6421625E-822.49854728
ATCGAGA500.002264653322.49854744
AGAGCGG1257.4760464E-1021.607575
TCTCGTA1056.1041646E-821.42718741
GAGACCG1109.9780664E-820.45322627
Produced by FastQC (version 0.11.5)