FastQCFastQC Report
Mon 6 Jun 2016
ERR1379074.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379074.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665194
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC950.040.26073530
CCGGACG900.039.99759331
CGGACGG1000.035.99783332
TCTCGTA1100.034.77063441
GACCGGA1150.033.2588729
AGACCGG1150.033.2588728
CGGTTCA1250.032.3980529
GCGGTTC1200.031.8778768
ATCTCGT1200.031.87308140
CCGAGAC1200.031.87308125
CTCGTAT1250.030.5981642
GAGCGGT1300.029.4301576
TCGTATG1300.029.42130943
CGGAATC1250.028.7982736
AGCGGTT1350.028.3358927
GAGACCG1350.028.33162927
AGAGCGG1400.027.3280035
CGTATGC1400.027.31978644
GGACGGA1350.026.66506433
TGCCGAG1450.026.37772423
Produced by FastQC (version 0.11.5)