FastQCFastQC Report
Mon 6 Jun 2016
ERR1379073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516432
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC401.5600343E-533.7464459
CCGAGAC754.7293724E-1132.99651725
ATCTCGT855.456968E-1231.76135840
CGTATGC801.0004442E-1030.9342444
CTCGTAT801.0004442E-1030.9342442
CGCGAGA801.0004442E-1030.9342432
TCTCGTA801.0004442E-1030.9342441
GCGGTTC852.0372681E-1029.1145788
ACCGCGA852.0372681E-1029.11457830
GACCGCG852.0372681E-1029.11457829
AGCGGTT852.0372681E-1029.1145787
GAGACCG852.0372681E-1029.11457827
AGACCGC852.0372681E-1029.11457828
AATCTCG852.0372681E-1029.11457839
CCGCGAG852.0372681E-1029.11457831
GCGAGAA903.947207E-1027.497133
AATGCCG903.947207E-1027.497121
CGCGCCC450.001226393324.99736411
CGGAAGA1001.3296813E-924.7689761
AAGAGCG1001.3296813E-924.7665754
Produced by FastQC (version 0.11.5)