FastQCFastQC Report
Mon 6 Jun 2016
ERR1379069.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379069.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746546
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA250.002107190435.9980742
GACCGTT606.4283086E-933.7481929
AGACCGT651.4029865E-831.15217828
ACCGTTC651.4029865E-831.15217830
GAGACCG702.8849172E-828.92702127
CGTATGC608.189927E-626.24859444
CTCGTAT608.189927E-626.24859442
TCTCGTA608.189927E-626.24859441
ATCTCGT802.9738203E-622.49879540
CGTTCTC1003.6421625E-822.49879532
CCGTTCT903.2854405E-722.49879531
TCGTATG754.6017587E-520.99887543
ATCGGAA550.003936487720.45344719
CCGAGAC1051.4449233E-619.28468125
CGGAAGA1102.2448949E-618.4167391
AGCGCTC1002.0460166E-517.9990357
GAGCGGT1153.436151E-617.6136516
GCGCTCC1153.4471486E-617.6077528
CGCCACT901.865336E-417.4990629
GCGGTTC1205.170845E-616.8740968
Produced by FastQC (version 0.11.5)