FastQCFastQC Report
Mon 6 Jun 2016
ERR1379062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773434
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAA250.00210693635.9991239
CGTTCGA406.1651866E-428.12431332
GATCGGA507.156613E-526.9993418
AGACCGT507.156613E-526.9993428
ATCGCGC450.001225442225.0026235
GCGCTTT450.001226366324.9993935
CCGTTCG450.001226366324.9993931
GATCGCG500.002262565822.502364
ATCTCGT602.4669047E-422.4994540
AGATCGG602.4669047E-422.4994517
CCGGCTA754.601E-520.99948716
TCTCGTA550.00393594920.45404641
ACCGTTC550.00393594920.45404630
AGAGCGA807.560333E-519.6870235
CGTATGC706.9817883E-419.28524444
AGCGCTC750.001109959117.999567
CGCCACT901.8650295E-417.4995739
CGCCACC1205.163536E-616.876775
ATCGGAA800.001710441516.87458819
GCGACAG952.818385E-416.57854330
Produced by FastQC (version 0.11.5)