FastQCFastQC Report
Mon 6 Jun 2016
ERR1379060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910969
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC453.4905213E-529.99941644
GACCGGC453.4905213E-529.99941629
GCGAGAG602.463626E-422.5045011
CTCGTAT602.46729E-422.49956142
GGGCGAC955.5301643E-721.31537428
CGGTTCA754.60198E-520.999599
TCTCGTA654.2378608E-420.76882641
TGGGCGA1151.5947626E-719.56483527
ATCTCGT706.982864E-419.2853440
ACCGGCC706.982864E-419.2853430
TCGTATG706.982864E-419.2853443
GCGGTTC851.2042732E-418.529058
CCGAGAC750.001110128617.9996525
AGCGGTT901.8654189E-417.4996597
CGGCCTT800.001710700316.87467232
AGACCGG800.001710700316.87467228
GGCGACA1205.171689E-616.8746729
GAGCGGT952.8166096E-416.5804446
CGAGGTG850.002562134515.8846592
GAGACCG850.002564951315.88204327
Produced by FastQC (version 0.11.5)