FastQCFastQC Report
Mon 6 Jun 2016
ERR1379059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701635
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT800.039.37350529
ACCGATC800.039.37350530
CCGATCC850.037.05741531
GAGACCG900.034.9986727
CGTATGC850.034.41045444
AGACCGA950.033.15663528
CTCGTAT901.2732926E-1129.9988642
TCTCGTA901.2732926E-1129.9988641
TCGTATG952.3646862E-1128.41997343
GTATGCC1053.6379788E-1227.85608545
CGGTTCA1150.027.3902659
AGCGGTT1150.027.3902657
CGATCCT1150.027.39026532
ATCTCGT1004.5474735E-1126.99897640
GATCCTA1200.026.24933
AATGCCG1200.026.24921
ATGCCGA1200.026.24922
AATCTCG1058.367351E-1125.71330839
AGAGCGG1251.8189894E-1225.2044325
GCGGTTC1251.8189894E-1225.1990418
Produced by FastQC (version 0.11.5)