FastQCFastQC Report
Mon 6 Jun 2016
ERR1379057.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379057.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720370
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC501.9795225E-631.50150744
AGACCGA501.9805957E-631.4993228
TCGTATG554.1605526E-628.63773243
ACCGACA554.162801E-628.63574430
CGGTTCA608.187371E-626.2494359
AGCGGTT608.187371E-626.2494357
GAGCGGT608.187371E-626.2494356
CGACAGT651.5229798E-524.23024632
CTCGTAT702.7001863E-522.50107442
AGAGCGG702.7001863E-522.5010745
GGTTAGC500.002264079622.49951413
TCTCGTA754.597877E-521.00100341
GCGGTTC754.600337E-520.9995448
CGGAAGA951.3133893E-518.949591
GACCGAC901.8647657E-417.49962229
CCGACAG901.8647657E-417.49962231
TCGTTGG1601.6094418E-816.8758074
AGAAACG800.00171025516.8746368
ATCTCGT952.8164947E-416.5797440
AACGGGA1652.354318E-816.36328312
Produced by FastQC (version 0.11.5)