FastQCFastQC Report
Mon 6 Jun 2016
ERR1379056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729665
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGAGATCTCGTATGCC12000.16445903256974093Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT1600.040.78069329
CGGTAGA1650.039.54491432
TCTCGTA1600.039.37446241
GAGACCG1550.039.19301227
AGCGGTT1700.038.3818247
CCGGTAG1700.038.38182431
CTCGTAT1650.038.18129742
TCGTATG1650.038.18129743
AGACCGG1750.037.28520228
GCGGTTC1900.036.7100228
ACCGGTA1800.036.24950430
CCGAGAC1700.035.73480225
CGGAAGA1850.035.2746241
GAGCGGT1850.035.2722056
CGTATGC1800.034.9995244
AAGAGCG1900.034.343994
CGGTTCA2050.032.9263769
AGAGCGG2000.032.626795
CGACATG300.00514293730.00371
CTAATCG300.00514638529.9995939
Produced by FastQC (version 0.11.5)