FastQCFastQC Report
Mon 6 Jun 2016
ERR1379055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945252
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT1351.9535946E-920.0074332
CCTTCGT1202.4857582E-718.7569681
GGGCGCC600.006511605318.74903114
GGCGCCT1053.1128577E-517.14197215
TCGTATA850.002565597615.8815329
GCGCCTG1004.1677043E-415.74918516
TCGTTGG1654.184858E-715.0039864
CGTATAA900.003754555714.99922510
TTCGTTG2001.564149E-814.6304343
CGTTGGA1705.8667683E-714.5626935
CTTCGTA1251.3800278E-414.4000177
ACGCCCG1108.589717E-414.31744212
CGCCACC1453.0954514E-513.9692295
GATTTCG1000.007549655613.5000157
GCCTAAC1000.00755229813.49930145
CCCGCCA1200.001651402113.1298773
ATCTTCG1200.001652718613.1284895
CGCCCGG1403.6069742E-412.85647813
AAAACGA1454.8492267E-412.41315116
AAACGGG1852.2431446E-512.16153311
Produced by FastQC (version 0.11.5)