FastQCFastQC Report
Mon 6 Jun 2016
ERR1379054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687539
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAC501.0804797E-940.4984325
AGAGCGG659.094947E-1238.083765
GACCGGC601.546141E-1037.49854329
TCTCGTA601.546141E-1037.49854341
AGACCGG601.546141E-1037.49854328
GAGCGGT653.6561687E-1034.6215976
CCGCGAT653.6743586E-1034.61404435
AGATCGC352.813836E-432.1533053
CTCGTAT708.1308826E-1032.1416142
AGCGGTT708.1308826E-1032.141617
CGTATGC651.4024408E-831.1526444
GAGACCG651.4024408E-831.1526427
AAGAGCG751.6971171E-930.0053834
GCGGTTC751.7025741E-929.9988358
GCCGCGA751.7025741E-929.99883534
ACCGGCC852.0372681E-1029.11651830
TCGTATG702.8838258E-828.92744843
CGGAAGA856.4501364E-926.4791891
CCGGCCG856.4737833E-926.46956331
ATGCCGA856.4737833E-926.46956322
Produced by FastQC (version 0.11.5)