FastQCFastQC Report
Mon 6 Jun 2016
ERR1379052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951517
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA453.8562575E-1044.99721532
CCGCACG501.0822987E-940.49749431
GAGACCG601.5643309E-1037.4976827
CGTATGC606.4337655E-933.74791344
GACCGCA606.4337655E-933.74791329
AGACCGC606.4337655E-933.74791328
CTCGTAT708.1490725E-1032.14086542
TCGTATG651.403896E-831.15191743
CGATATC651.403896E-831.15191736
TATCTCG702.886918E-828.9267839
CACGATA755.6403223E-826.9983334
ACGATAT755.6403223E-826.9983335
ACCGCAC801.0540316E-725.31093230
TCTCGTA901.1912562E-824.99845341
AGAGCGG903.2760363E-722.5068875
GCGGTTC903.284331E-722.5009738
CCGAGAC903.2876596E-722.49860825
ATGCCGA1003.645073E-822.49860822
GCACGAT955.5329656E-721.3144733
GCGCTCC855.0288236E-621.1773858
Produced by FastQC (version 0.11.5)