FastQCFastQC Report
Mon 6 Jun 2016
ERR1379050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028221
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCCG950.035.52516630
CCGCCGT1050.034.28460331
GACCGCC1050.032.14181529
TGGCGCG855.456968E-1231.76367612
TCTCGTA1000.031.50051141
AGACCGC1100.030.68082228
TGTACCG300.00514782629.99902526
CTCGTAT1001.8189894E-1229.25047542
GCGCGAT852.0372681E-1029.11670314
GGCGCGA852.0372681E-1029.11670313
GCGGTTC1250.028.7990658
CCGTCCA1200.028.12408634
CGTATGC1053.6379788E-1227.85759544
CGCCGTC1300.027.6914132
GCCGTCC1300.027.6914133
CGCGATC903.965397E-1027.4991115
AGAGCGG1350.026.6696935
CGTCCAT1300.025.96069735
GAGCGGT1400.025.7159526
CGGTTCA1500.025.4991749
Produced by FastQC (version 0.11.5)