FastQCFastQC Report
Mon 6 Jun 2016
ERR1379046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927255
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT602.415327E-730.0039566
AGACCGC608.19122E-626.24921628
AGCGGTT709.302348E-725.7149037
TCGTATG702.7028897E-522.49932743
CTCGTAT754.6024626E-520.9993742
CCGATGC550.00393665620.45393238
GCGGTTC908.246716E-620.0004798
CGGTTCA908.250561E-619.9994019
CCGCTCA807.562727E-519.6869131
CGTATGC951.3157236E-518.946844
GACCGCT851.2043994E-418.52885629
AATGCCG851.2043994E-418.52885621
TCTCGTA901.8656136E-417.49947541
AGAGCGG1104.6307297E-516.3657935
ACCGCTC1004.1669077E-415.74952830
CGCTCAG1004.1669077E-415.74952832
AAGAGCG1056.029265E-415.0019774
ATCTCGT1056.0366717E-414.99955140
AATCTCG1108.588086E-414.31775339
GCGCTCC950.00537412314.2108678
Produced by FastQC (version 0.11.5)