FastQCFastQC Report
Mon 6 Jun 2016
ERR1379043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628359
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT403.4561708E-739.3731242
CGTACAA250.002106877535.9982831
GACCGAA458.694824E-734.9983329
TCTCGTA458.694824E-734.9983341
GCGGTTC602.4170367E-729.998578
TATCTCG602.4170367E-729.9985739
CGTATGC554.1625353E-628.63499644
GAGCGGT654.8627226E-727.6953936
CGAAGAC507.15565E-526.99870932
AGACCGA608.186858E-626.24874928
CGGTTCA709.3001654E-725.7130589
CCGAAGA551.367682E-424.54428331
GAGACCG651.5228861E-524.2296127
TCGTATG651.5228861E-524.2296143
AGCGGTT751.6963095E-623.9988547
AGAGCGG855.017908E-621.1805155
ACCGAAG855.028298E-621.17545930
ATCTCGT754.600062E-520.99899740
CCGAGAC654.2366504E-420.76823825
GACTATC654.2366504E-420.76823836
Produced by FastQC (version 0.11.5)