FastQCFastQC Report
Mon 6 Jun 2016
ERR1379036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813644
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT803.6379788E-1233.74810842
TCTCGTA803.6379788E-1233.74810841
CGTATGC952.3646862E-1128.41946244
AGCGGTT1004.5474735E-1127.0001497
AGACCGA1004.5474735E-1126.99848728
TCGTATG1004.5474735E-1126.99848743
GCCGATC957.421477E-1026.05117236
ATGCCGA957.421477E-1026.05117222
GCGGTTC1058.367351E-1125.7144268
GACCGAG1058.54925E-1125.71284529
GAGCGGT1159.094947E-1225.4411756
ATCTCGT1101.5097612E-1024.5440840
TGCCGAG1101.5097612E-1024.5440823
CGGAAGA1201.6370905E-1124.3856241
GGCCGAT1052.3665052E-923.57010835
AGAGCGG1152.6375346E-1023.4841615
CGGTTCA1152.6557245E-1023.4769469
ACCGAGG1152.6557245E-1023.47694630
AAGAGCG1204.4929038E-1022.5056554
AATGCCG1104.0508894E-922.49874121
Produced by FastQC (version 0.11.5)