FastQCFastQC Report
Mon 6 Jun 2016
ERR1379035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480469
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT302.163406E-644.99833742
TCTCGTA302.163406E-644.99833741
CCGACCG302.163406E-644.99833731
GACCGAC356.242286E-638.5729
ACCGACC356.242286E-638.5730
AAGCTCG250.00210412236.0061654
CGCTGTA250.00210627335.9986745
GAGCGGT401.5568583E-533.7557756
AGAGCGG401.5568583E-533.7557755
AGACCGA401.559079E-533.74875328
CCGAGAC501.9790168E-631.49883725
GCGGTTC453.4875404E-529.9988928
AGCGGTT453.4875404E-529.9988927
CGGAAGA507.131432E-527.0102461
CGGGGTT1004.5474735E-1127.0102461
ACCGGCA507.151677E-526.99900234
CGTATGC507.151677E-526.99900244
CGGTTCA507.151677E-526.9990029
CGACCGG507.151677E-526.99900232
AATGCCG507.151677E-526.99900221
Produced by FastQC (version 0.11.5)