FastQCFastQC Report
Mon 6 Jun 2016
ERR1379034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757759
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG1454.7584763E-918.6227384
CTTCGTA851.20395634E-418.5290057
TCTTCGT1102.251547E-618.4111186
CCTTCGT1354.2578904E-818.3365611
TCGTATA750.001109912817.9996039
ATCTTCG1153.4419136E-617.6106325
CGTATAA800.001710370916.87462810
CTTCGTT1401.2510172E-616.0742572
TTCGTTG1602.9531657E-715.4714723
CGTTGGA1754.84888E-815.4302685
ACCACGC1056.034503E-414.999679
TCGCTTG1150.001201315813.69535215
ACTAGAC3550.013.309566519
TATCTTC6100.012.5423624
ATATCTT6750.012.0021113
GAAACGG2158.19657E-611.51137510
GGAAACG2158.19657E-611.5113759
GCTAGAC1802.3425817E-411.24975333
ACGGGAT1450.006822047310.86183113
AGAATCG1450.006822047310.86183111
Produced by FastQC (version 0.11.5)