FastQCFastQC Report
Mon 6 Jun 2016
ERR1379033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271251
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT1700.029.11632340
CTCGTAT1800.027.4987542
ACCGAGT2050.027.43885630
GCGGTTC2000.026.9998368
CGGTTCA2000.026.9987749
CATCTCG1850.026.7555439
GACCGAG2050.026.34130129
TCTCGTA1950.025.38346141
GAGCGGT2150.025.119096
CGTATGC2000.024.74887744
AGACCGA2200.024.54530328
AAGAGCG2400.024.3796444
TCGTATG2050.024.14524343
CGGAAGA2250.024.0074081
AGAGCGG2250.024.0045745
GAGACCG2250.023.99985527
CCGAGAC2200.023.52258325
ATGCCGA2250.022.99895522
AGCGGTT2350.022.9785847
CGAGTTC2350.022.9776832
Produced by FastQC (version 0.11.5)