FastQCFastQC Report
Mon 6 Jun 2016
ERR1379029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168129
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC751.7043931E-929.99945644
ATCTCGT803.3960532E-928.1244940
TCGGATA406.166858E-428.1244927
TCGTATG803.3960532E-928.1244943
AGACCGC856.4828782E-926.4701128
TCACGAC450.001226695824.99954828
GACCGCC851.8940409E-723.823129
ACGCCCG500.002264869622.49959412
CTCGTAT1003.6452548E-822.49959442
AGCGGTT1104.0545274E-922.4995947
TCTCGTA1003.6452548E-822.49959441
GCGGTTC1056.109258E-821.4281858
CCGCCAA1056.109258E-821.42818531
ACCGCCA955.5328564E-721.31540530
CGGTTCA1151.5957085E-719.5648639
CGGAAGA1302.7661372E-819.0421921
GAGCGGT1302.7703209E-819.0397476
GAGACCG1202.4972178E-718.74966227
AATCTCG1551.09030225E-817.41903939
CCACGCC1953.45608E-1117.3073810
Produced by FastQC (version 0.11.5)