FastQCFastQC Report
Mon 6 Jun 2016
ERR1379023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617836
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT45930.2838977498337285Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTAAGAATCTCGTATGCCGTC26700.1650352693350871Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG250.002107617235.9991138
TATGCCG4300.030.350943
ATCGGAA6900.029.67226227
GATCGGA7000.029.56978226
AGATCGG7100.029.47019225
TCGGAAG7100.029.47019228
ATGCCGT4600.028.37149644
TGCCGTC4700.027.76784745
AATGCCG12900.026.68456318
ATGCCGA13650.025.38320419
TGCCGAG13600.025.31109220
GACCGTT5250.024.85575926
ACCGTTA5300.024.19676627
CGGAAGA9100.022.99322929
CTCGTAT5850.022.69174639
GAATGCC15600.022.49874917
CGTTAAG5700.022.49874929
AGACCGT5800.022.49874725
CCGTTAA5800.022.49874728
TCTCGTA5950.022.31037338
Produced by FastQC (version 0.11.5)