FastQCFastQC Report
Mon 6 Jun 2016
ERR1379022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278113
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT65440.5120048070866973Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCCGTC39590.30975351944624613Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAACAATCTCGTATGCC23880.18683793999435105Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5300.038.2146943
ATGCCGT5800.034.92032244
ATCGGAA8000.034.59209427
GATCGGA8100.034.1650326
TCGGAAG8450.033.2811328
TGCCGTC6100.032.83400345
AGATCGG8950.030.9203125
TGCCGAG16600.028.86797320
AATGCCG16700.028.82982818
CCGAGAT9650.028.67738522
ATGCCGA16850.028.57318319
CGAGATC9950.028.03885823
CGATAAC7250.027.92860829
ACCGATA7450.027.17990927
CCGATAA7550.026.8188628
CTCGTAT7300.026.81483539
CGTATGC7450.026.57903141
GAATGCC18400.026.5330217
GCCGAGA18000.026.3737421
GACCGAT7550.026.2239126
Produced by FastQC (version 0.11.5)