FastQCFastQC Report
Mon 6 Jun 2016
ERR1379020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267787
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT49340.38918209446855034Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTGAAATCTCGTATGCCGTC26960.21265401838005912Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3750.037.80417643
GATCGGA6350.034.36845426
ATCGGAA6400.034.0999527
TCGGAAG6550.033.6625328
AGATCGG6750.032.33180625
AATGCCG11300.031.85815418
ATGCCGT4550.031.15728844
TGCCGAG11800.030.8883720
TGCCGTC4650.030.48723845
GACCGAT4700.030.1581526
ACCGATT4700.030.1581527
ATGCCGA12000.029.99976319
CCGATTG4850.029.22542228
CGGAAGA7650.028.82216629
GCCGAGA12550.028.68390321
CGTATGC5050.028.0701941
CTCGTAT5150.027.95984339
CCGAGAT7900.027.91007222
TCGTATG5200.027.69099840
AGACCGA5200.027.25832625
Produced by FastQC (version 0.11.5)