FastQCFastQC Report
Mon 6 Jun 2016
ERR1379018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359155
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT59500.4377719980429016Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAACAAATCTCGTATGCCGTC34730.2555264116307559Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAACAAATCTCGTATGCC21550.15855439592982404Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10150.036.7956526
ATCGGAA10100.036.75505427
AGATCGG10350.036.30200625
TCGGAAG10600.035.87032728
TATGCCG4700.033.51217343
GAGATCG11450.033.20746224
CCGAGAT11950.032.006322
AATGCCG17500.031.49796518
CGAGATC12000.031.49796523
CGGAAGA12200.031.16602129
TGCCGAG17900.030.79410220
ATGCCGT5200.030.72256344
ATGCCGA17950.030.70832319
GAATGCC19250.028.86826517
GCCGAGA19050.028.46272921
GGAATGC20600.027.08562916
TGCCGTC5900.026.69613845
GACCGTA5900.026.69319226
ACCGTAA6000.026.24830427
TCTCGTA6050.025.66044438
Produced by FastQC (version 0.11.5)