FastQCFastQC Report
Mon 6 Jun 2016
ERR1379017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307729
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT49590.37920700695633425Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCCGTC28950.22137614138709166Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCC17180.13137278442246061Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3400.035.736643
AGATCGG7050.035.10363825
GATCGGA7050.034.78451526
ATCGGAA7150.033.98335627
TCGGAAG7450.032.9168928
TGCCGTC3950.031.3302645
ATGCCGT4100.030.1840344
ATGCCGA12300.030.18056919
AATGCCG12700.029.05284718
TGCCGAG13050.028.27365320
CCGAGAT9050.027.34592822
GAGATCG9150.026.80118424
CGGAAGA9150.026.555329
ACCGATT4600.025.92188627
CCGATTA4700.025.37035828
GCCGAGA14500.025.29112621
CGATTAA4750.025.103329
CGAGATC10050.025.07266823
CGTATGC4850.025.05150241
GAATGCC14950.024.83083517
Produced by FastQC (version 0.11.5)