FastQCFastQC Report
Mon 6 Jun 2016
ERR1379016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098894
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT47210.42961377530498845Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTATGCCGTC25780.2345995155128702Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTATGCC16340.14869496056944528Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3200.038.67496543
ATGCCGT3450.035.8724344
GATCGGA5950.032.89729726
TCGGAAG5950.032.89729728
ATCGGAA5900.032.79475427
AGATCGG6050.031.9816625
TGCCGTC4000.030.9399745
TGCCGAG11400.028.81415720
AATGCCG11350.028.54463818
ATGCCGA11750.027.95586419
CGAGATC7350.026.63114523
CGAGTAA4650.026.61139729
CGGAAGA7450.026.2736829
CGTATGC4650.026.1299341
ACCGAGT4750.026.05115727
CCGAGAT7650.025.88088822
TCGTATG4700.025.849640
GAGATCG7650.025.58678624
CTCGTAT4850.025.51401939
TCTCGTA4800.025.31106838
Produced by FastQC (version 0.11.5)