FastQCFastQC Report
Mon 6 Jun 2016
ERR1379014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276397
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT61130.47892622749818436Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGTAATCTCGTATGCCGTC35500.27812663301464985Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCGTAATCTCGTATGCC19680.15418400387967066Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG207.035812E-444.997124
TATGCCG4900.037.1987743
ATGCCGT5300.034.815944
TGCCGTC5250.034.7188545
GATCGGA8200.032.65033326
AGATCGG8300.032.25695825
ATCGGAA8350.032.063827
TCGGAAG8500.031.76266128
AATGCCG16450.027.7641718
CTCGTAT6900.027.06771539
ATGCCGA16800.026.91790819
TGCCGAG16900.026.89175820
CCGAGAT10150.026.8209322
CGTATGC6800.026.80394441
GCGTAAT6850.026.60412632
GAGCGTA6900.026.41134330
CGTAATC7000.026.03403733
TCGTATG7100.025.98831440
CGAGCGT7050.025.5302729
GTATGCC7200.025.31582842
Produced by FastQC (version 0.11.5)