FastQCFastQC Report
Mon 6 Jun 2016
ERR1379013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914290
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT68300.7470277483074298Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCCGTC46170.504982007896838Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGAGTCATCTCGTATGCC15890.1737960603309672Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9720.1063120016624922Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5650.039.83138343
TGCCGTC6100.036.89300545
ATGCCGT6150.036.22712744
GATCGGA9300.036.0461126
ATCGGAA9450.035.4739527
TCGGAAG9950.034.59580628
AGATCGG9700.034.5596725
AATGCCG18800.030.6363618
CCGAGAT11100.030.60617322
CGAGATC11050.030.54105823
ATGCCGA19400.029.57287219
GAGATCG11450.029.4741224
TGCCGAG19650.029.1966320
TCGTATG7600.029.01449840
CTCGTAT7700.028.92990939
GAATGCC20050.028.72793417
CGTATGC7750.028.45292741
CGGAAGA12200.028.03101529
GCCGAGA20150.028.02552421
TCTCGTA7900.027.91268238
Produced by FastQC (version 0.11.5)