FastQCFastQC Report
Mon 6 Jun 2016
ERR1379012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232142
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT66090.5363829818316396Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCCGTC35330.2867364313528798Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGTAATCTCGTATGCC15210.12344356413465331Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.033.75435343
ATGCCGT4400.031.70863344
GATCGGA9100.030.4098426
ATCGGAA9100.030.4098427
AGATCGG9250.030.1599325
TCGGAAG9500.030.07672128
TGCCGTC4600.029.84080345
CGGCAGT5100.028.23320629
ATGCCGA15500.028.01405719
AATGCCG15750.027.5693918
TGCCGAG15700.027.5138920
CTCGTAT5050.027.17730939
CGTATGC5100.026.46970441
TCTCGTA5200.026.3933538
CCGAGAT10700.026.0728422
CGGAAGA11050.025.85781729
AGACCGG5600.025.71238525
CGAGATC10850.025.71238323
GAGATCG10950.025.4775724
TCGTATG5400.025.4158240
Produced by FastQC (version 0.11.5)