FastQCFastQC Report
Mon 6 Jun 2016
ERR1379011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095565
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT71370.6514446883571491Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGATGATCTCGTATGCCGTC43760.3994286053314956Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGATGATCTCGTATGCC13700.12504963192507976Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5450.036.74617844
TATGCCG5350.036.5918343
GATCGGA10300.035.1680126
ATCGGAA10300.035.1680127
TGCCGTC5700.034.3449645
AGATCGG10800.033.74818425
TCGGAAG11200.033.1455428
AATGCCG18100.031.07567618
ACCGGTG6400.030.93583527
GACCGGT6400.030.93583526
CCGAGAT11900.030.62860722
ATGCCGA18450.030.48616419
CTCGTAT6450.030.34997739
TGCCGAG18700.030.31922520
TCTCGTA6550.029.88661838
GAGATCG12300.029.81547224
AGACCGG6650.029.77283525
CGGAAGA12500.029.69840229
TCGTATG6600.029.66020440
CGTATGC6650.029.43719541
Produced by FastQC (version 0.11.5)