FastQCFastQC Report
Mon 6 Jun 2016
ERR1379009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018046
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT48520.4765992892266165Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCCGTC28510.280046284745483Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCC13570.1332945662573204Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3800.034.939443
ATCGGAA7250.033.5139927
GATCGGA7450.032.91626426
ATGCCGT4100.032.3828644
AGATCGG7650.032.349825
TCGGAAG7650.032.0557128
TGCCGTC4150.031.992745
AATGCCG13500.028.66388318
GAGATCG8850.027.96338524
ATGCCGA13850.027.61464519
CGAGATC9150.027.29242923
TGCCGAG14100.027.28458420
CGGAAGA9250.026.51093929
CCGAGAT9500.026.2869222
CTCGTAT5150.025.77799239
GAATGCC15300.025.14461717
CCGGTGT5200.025.09371828
GACCGGT5300.024.62024926
ATCTCGT5400.024.58456837
TCGTATG5400.024.58456840
Produced by FastQC (version 0.11.5)