FastQCFastQC Report
Mon 6 Jun 2016
ERR1379005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090963
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT41990.3848893133864302Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCCGTC24960.22878869402537025Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCGAATCTCGTATGCC11020.10101167500639342Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG6200.034.10991728
ATCGGAA6150.034.02140827
GATCGGA6450.032.78782726
AGATCGG6700.031.56439825
CCGAGAT7250.028.8595422
TATGCCG3300.028.64435843
AATGCCG10900.028.07093618
TGCCGTC3400.027.80187645
CGGAAGA7700.027.75730929
ATGCCGA11000.027.4066919
ATGCCGT3450.027.39895244
TGCCGAG11050.027.28267720
GAGATCG7750.026.99763524
CGAGATC7900.026.48502223
CGTATGC3800.025.46413241
GCCGAGA12000.025.31028221
CGAATCT3900.024.80552134
GACCGGA3900.024.22864726
GAATGCC12850.024.16130817
CTCGTAT4000.023.62509539
Produced by FastQC (version 0.11.5)