FastQCFastQC Report
Mon 6 Jun 2016
ERR1379004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689274
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT64230.9318500335135229Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCCGTC32580.47267124539733113Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCC18510.2685434239504174Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7880.11432318642513718Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG9200.038.88110728
GATCGGA9250.038.4277226
ATCGGAA9400.037.81451427
AGATCGG9600.037.02671425
CCGAGAT10050.035.81650522
TGCCGTC3800.034.95021445
TATGCCG3850.034.49381343
GAGATCG10450.034.23025524
CGAGATC10600.033.95810323
ATGCCGT4150.033.62741544
AATGCCG15050.032.88633718
ATGCCGA15250.032.4550419
CGGAAGA11400.031.57507929
TGCCGAG15850.031.22645620
GAATGCC16100.030.74380717
GCCGAGA15950.030.60753421
GCGGACG300.00514892829.99632531
GGAATGC16900.029.28847716
AGGAATG18350.027.09673515
GAAGAGC13500.026.9966931
Produced by FastQC (version 0.11.5)