FastQCFastQC Report
Mon 6 Jun 2016
ERR1379003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801403
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT69080.8619882880398502Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCCGTC35140.43848101392183453Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAGCATCTCGTATGCC13700.17095019609360085Illumina Paired End PCR Primer 2 (97% over 34bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9630.1201642619256479Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4450.037.42870343
ATGCCGT4750.035.53863544
TGCCGTC5000.033.76170345
GATCGGA9350.033.44618226
AGATCGG9450.032.85418325
ATCGGAA9700.032.47130227
TCGGAAG10100.032.07631728
CCGAGAT10200.030.65922
CGAGATC10300.030.36134123
GAGATCG10300.030.36134124
AATGCCG16800.028.65813318
ATGCCGA16950.028.5372519
TGCCGAG16950.028.4045220
CGTATGC6000.027.38107541
CGGAAGA11950.027.29879229
GCCGAGA17350.027.23097621
AGACCGA6050.027.14631325
TCTCGTA6150.027.0741138
GAATGCC18150.027.0223617
CTCGTAT6150.026.70824439
Produced by FastQC (version 0.11.5)