FastQCFastQC Report
Mon 6 Jun 2016
ERR1379002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821393
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT65790.8009564240260144Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCCGTC43450.5289794288483102Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGAGAATCTCGTATGCC11250.136962452808826Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC10090.12284010211920482Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5150.036.26498443
ATGCCGT5150.036.26498444
TGCCGTC5400.034.5860545
ATCGGAA7300.033.9026427
GATCGGA7450.033.2200426
TCGGAAG7600.033.1564628
AGATCGG7650.032.64564525
CCGTCGA5900.031.65117328
AATGCCG13950.031.45025318
CGTATGC5950.031.38710641
CTCGTAT5950.031.38519739
AGACCGT5850.031.15249825
CGAGAAT6000.031.12365232
GACCGTC5950.031.00706126
TCTCGTA6050.030.86643238
ACCGTCG6050.030.86643227
TGCCGAG14300.030.83782620
GTCGAGA6150.030.73037730
GTATGCC6150.030.36823842
ATGCCGA14450.030.36200719
Produced by FastQC (version 0.11.5)