FastQCFastQC Report
Mon 6 Jun 2016
ERR1379001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128151
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT57590.510481309682835Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACGAGTATCTCGTATGCCGTC31930.28302948807384826Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGAGTATCTCGTATGCC18530.16425106213618568Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4050.038.340743
TGCCGTC4550.033.63283545
GATCGGA8000.032.6225926
AGATCGG8200.032.10128825
ATCGGAA8150.032.02217527
ATGCCGT4900.031.23048844
TCGGAAG9000.029.99778628
CCGAGAT9150.029.0142522
ATGCCGA15200.027.82689319
AATGCCG15350.027.55496818
TGCCGAG15700.027.08398620
GAGATCG9650.027.04463624
GAATGCC16200.026.2480617
CGAGATC9950.026.2292223
GCCGAGA16550.025.28514121
CGGAAGA10700.025.2317829
ACGAGTA6050.024.91551631
TCTCGTA6200.024.31487538
GACGAGT6200.024.3127230
ACCGACG6300.024.2839227
Produced by FastQC (version 0.11.5)