FastQCFastQC Report
Mon 6 Jun 2016
ERR1379000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692477
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT57640.8323742160389443Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCCGTC33730.4870919900588756Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCC16020.23134342368049768Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7130.10296370854194435Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3900.037.5025343
ATGCCGT3850.037.40511744
ATCGGAA7450.036.2388227
TCGGAAG7600.035.81960728
TGCCGTC4050.035.55795345
GATCGGA7850.034.39225426
AGATCGG8000.034.0286325
CGAGATC8450.032.21645423
CCGAGAT8750.031.36901322
GAGATCG9150.029.9976924
TGCCGAG14500.028.85984820
AATGCCG14900.028.08508318
ATGCCGA15300.027.35083619
GCCGAGA15500.026.8527721
GAATGCC15600.026.82485617
CGGAAGA10350.026.08494829
GGAATGC16550.025.2850616
TATCTCG6100.023.60814936
AGGAATG18000.023.4981915
TCGTATG6300.023.21584940
Produced by FastQC (version 0.11.5)