FastQCFastQC Report
Mon 6 Jun 2016
ERR1378999.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378999.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049333
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT50800.48411705340440064Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCCGTC23920.227954329083332Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCC16040.15285900662611393Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.037.92921443
GATCGGA7000.034.391826
AGATCGG7300.033.28664825
ATCGGAA7250.033.20587527
ATGCCGT4050.032.7783344
TGCCGTC4050.032.7783345
TCGGAAG7600.032.5647928
CCGAGAT8350.029.63980922
CGAGATC8200.029.63323823
AATGCCG13400.028.8797118
ATGCCGA13450.028.7723519
TGCCGAG13550.028.72605320
GAGATCG8600.027.99332624
CGGAAGA9200.026.6567929
GCCGAGA14450.026.46977421
GAATGCC14850.026.05980517
CCGTGCA5350.025.2328728
CGTATGC5300.025.04759441
TCGTATG5300.025.04759440
AGACCGT5450.024.7698825
Produced by FastQC (version 0.11.5)