FastQCFastQC Report
Mon 6 Jun 2016
ERR1378996.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378996.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685655
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT75081.0950113395220629Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCCGTC48500.7073528232128402Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCC17360.2531885569273177Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC11600.16918129379935973Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5500.038.87491244
TGCCGTC5550.038.11916445
TATGCCG5700.037.11602843
ATCGGAA9200.036.437527
ACGGGTG250.002107288235.99731431
AGATCGG9450.035.71162425
GATCGGA9450.035.47354526
TCGGAAG9450.035.47354528
CCGAGAT10550.032.41464622
CGAGATC10500.032.3547323
GAGATCG10450.032.29424324
ATGCCGA17350.032.1589919
TGCCGAG17600.031.70218320
AATGCCG17750.031.56102618
CCGATAG6950.030.75310328
GAATGCC18200.030.65705517
TAGCGAT7000.030.5334432
GCCGAGA18250.030.44978721
ACCGATA7150.030.20753727
CTCGTAT7100.030.1077839
Produced by FastQC (version 0.11.5)